![]() Learn more about western blotting techniquesĪre you struggling with western blots? The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Once proteins are immobilized on a membrane, they are available for visualization, detection, and analysis. This involves two phases: protein transfer to a membrane and detection of the membrane-immobilized protein. The western blotting workflow involves the selection of appropriate methods, apparatus, membrane, buffer, and transfer conditions. This section taps into this expertise, providing an overview of western blotting methods and help with troubleshooting as well as information about products and solutions that will help you obtain the highest-quality western blotting data. With over 25 years of western blotting expertise, Bio-Rad provides a wealth of information and products to help optimize and troubleshoot western blotting. Western blotting combines the resolution of gel electrophoresis with the specificity of immunoassays, allowing the identification and analysis of individual proteins in complex samples. Western blotting, the transfer of proteins to a solid-phase membrane support followed by immunodetection, is a powerful and popular technique for the visualization and identification of proteins. Calculate the protein concentration of the test samples by comparing the OD595 with the standard curve(s), taking into account dilution factors (see figure Standard curve generated using the Bradford method).Complete Solutions for your Western Blotting WorkflowĮxplore Bio-Rad's complete solutions for your western blotting workflow - gels, electrophoresis chambers, western blotting transfer and imaging systems, buffers, membranes, and immunodetection reagents and kits. It is important that the protein samples to be tested are handled in exactly the same manner as the samples used in generating the standard curves.
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